Intranuclear growth of Rickettsia rickettsii

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Intranuclear growth of Rickettsia rickettsii.

The ability of the spotted fever agent, Rickettsia rickettsii, to grow not only in the cytoplasm but also in the nucleus of infected cells was first noted by Wolbach (12) in epithelial cells of the gut, hypoderm and salivary gland of infected ticks; it has subsequently been established for all spotted fever group rickettsiae investigated, especially when they were maintained on mammalian tissue...

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Plaque assay for Rickettsia rickettsii.

A plaque technique for the assay of Rickettsia rickettsii is described. The method employs primary chick or green monkey kidney monolayer cell cultures with either an agarose or special Noble agar overlay. Plaques were counted in 6 days and resultant titers correlated well with ld(50) end points obtained by a standard assay in embryonated eggs. Identification of the plaque-forming organisms was...

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Genomic comparison of virulent Rickettsia rickettsii Sheila Smith and avirulent Rickettsia rickettsii Iowa.

Rickettsia rickettsii is an obligate intracellular pathogen that is the causative agent of Rocky Mountain spotted fever. To identify genes involved in the virulence of R. rickettsii, the genome of an avirulent strain, R. rickettsii Iowa, was sequenced and compared to the genome of the virulent strain R. rickettsii Sheila Smith. R. rickettsii Iowa is avirulent in a guinea pig model of infection ...

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Rickettsia rickettsii in Amblyomma patinoi Ticks, Colombia

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Experimental Infection of Amblyomma aureolatum Ticks with Rickettsia rickettsii

We experimentally infected Amblyomma aureolatum ticks with the bacterium Rickettsia rickettsii, the etiologic agent of Rocky Mountain spotted fever (RMSF). These ticks are a vector for RMSF in Brazil. R. rickettsii was efficiently conserved by both transstadial maintenance and vertical (transovarial) transmission to 100% of the ticks through 4 laboratory generations. However, lower reproductive...

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ژورنال

عنوان ژورنال: Journal of Bacteriology

سال: 1968

ISSN: 0021-9193,1098-5530

DOI: 10.1128/jb.96.4.1415-1418.1968